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Flow cytometric analysis of the specific binding of nanobodies to cell-surface expressed PEDV S protein. The binding of nanobodies was detected using an APC-conjugated anti-mFc antibody (Y-axis), and S protein expression was verified using an anti-His FITC-conjugated antibody (X-axis). Cells in the upper right quadrant (Q2, FITC + /APC + ) represent the double-positive population, indicating successful binding of nanobodies to the expressed S protein. Positive control: Serum from PEDV-vaccinated pigs; negative control: an irrelevant nanobody

Journal: BMC Veterinary Research

Article Title: Neutralizing nanobodies against porcine epidemic diarrhea virus: discovery and characterization

doi: 10.1186/s12917-026-05628-z

Figure Lengend Snippet: Flow cytometric analysis of the specific binding of nanobodies to cell-surface expressed PEDV S protein. The binding of nanobodies was detected using an APC-conjugated anti-mFc antibody (Y-axis), and S protein expression was verified using an anti-His FITC-conjugated antibody (X-axis). Cells in the upper right quadrant (Q2, FITC + /APC + ) represent the double-positive population, indicating successful binding of nanobodies to the expressed S protein. Positive control: Serum from PEDV-vaccinated pigs; negative control: an irrelevant nanobody

Article Snippet: The FITC-conjugated goat anti-mouse IgG secondary antibody (catalog No. AS001, Abclonal, Wuhan, China) was used at a dilution of 1:500 for indirect immunofluorescence assay (IFA).

Techniques: Binding Assay, Expressing, Positive Control, Negative Control